Fig. 1: Assessment of the multilineage differentiation potential of miR-150-transfected ASCs. | Experimental & Molecular Medicine

Fig. 1: Assessment of the multilineage differentiation potential of miR-150-transfected ASCs.

From: A novel antifibrotic strategy utilizing conditioned media obtained from miR-150-transfected adipose-derived stem cells: validation of an animal model of liver fibrosis

Fig. 1

a Comparison of gross cell morphology of ASCs with/without miR-150 transfection. There was no difference in gross cell morphology between the two groups. b Flow cytometric analysis of ASCs transfected with miR-150. The miR-150-transfected ASCs were negative for CD31 and CD45 (hematopoietic stem cell markers) and positive for CD73 and CD105 (mesenchymal stem cell markers), and the expression pattern in ASCs without miR-150 transfection was identical to that of transfected ASCs. c Successful adipogenic [Top] and osteogenic [Bottom] differentiation of miR-150-transfected ASCs. Adipogenic and osteogenic differentiation were identified using Oil Red O and Alizarin red stains, respectively. d Successful chondrogenic differentiation of miR-150-transfected ASCs. Chondrogenic differentiation was identified using collagen types 1 and 2 and proteoglycan stains. Scale bars = 100 µm. The values are presented as the mean ± standard deviation of three independent experiments. *P < 0.05. ASC adipose-derived stem cell, t-ASC miR-150-transfected adipose tissue-derived stem cell.

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