Fig. 3: Fbp2 was important for cold tolerance during fasting conditions.

a Body temperature changes in WT and Fbp2 KO mice in cold stress experiments, n = 4 for the RT group; n = 8 for the cold group. b Glycogen content in the TA and QD muscles of WT and Fbp2 KO mice after cold stress experiments. n = 5 per group. c Glycogen content depending on cold exposure time (left) and glycogen content decrease after cold stress experiments (right) in the TA muscle of WT and Fbp2 KO mice. n = 5 per group. d Glycogen content in the QD muscle of WT and Fbp2 KO mice at room temperature. n = 5 per group. e Immunoblotting results of PKA substrate phosphorylation in WT and Fbp2 KO mice in cold stress experiments. n = 5 per group. f Gene expression analysis in the GAS muscle of WT and Fbp2 KO mice after cold stress. n = 4–6 per group. g Body temperature change, h body weight change, and i daily food intake of WT and Fbp2 KO mice in cold stress experiments for 3 weeks. n = 4–7 per group. h hour, TA tibialis anterior, QD quadriceps, PKA protein kinase A, Pygm glycogen phosphorylase, muscle associated, G6pi glucose-6-phosphate isomerase, Pfk1 phosphofructokinase 1, Pgm2 phosphoglucomutase 2, Gys1 glycogen synthase 1. Data are expressed as the mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 by Student’s t-test. ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001 by two-way ANOVA with post hoc analysis for a. ns nonsignificant.