Fig. 2: Knockdown of FliI promotes TG-induced apoptosis in CT26 cells.

Cell viability of FliI-KD CT26 cells was measured by MTT assay (a), cell counting assay (b), and colony assay (c) after treatment with TG for 48 h. Data are shown as the mean ± SEM. **p < 0.01; ***p < 0.001 shRNA-Ctrl cells vs. shRNA-FliI cells. After Ctrl- and FliI-KD cells were treated with TG for 48 h, they were subjected to annexin V-PI flow cytometry assay (d) and western blotting for PARP-1, cleaved caspase-3, FliI, and tubulin (e). TG thapsigargin.