Fig. 4: Knockdown of FliI increases the intracellular Ca2+ concentration in CT26 cells. | Experimental & Molecular Medicine

Fig. 4: Knockdown of FliI increases the intracellular Ca2+ concentration in CT26 cells.

From: Flightless-1 inhibits ER stress-induced apoptosis in colorectal cancer cells by regulating Ca2+ homeostasis

Fig. 4

Ctrl- and FliI-KD cells were incubated with Fluo-4AM for 30 min, and intracellular Ca2+ was (a) imaged on a fluorescence microscope (200×) and (b) measured by FACS analysis. c Ctrl- and FliI-KD cells were transfected with mCherry-GCaMP6s and imaged on a fluorescence microscope. Intracellular Ca2+ levels were monitored based on the ratio of GCaMP6s to mCherry fluorescence intensity. d Ctrl- and FliI-KD cells were pretreated with BAPTA (10 μM) and treated with TG (100 nM) for 48 h, and then apoptosis was detected by annexin V-PI flow cytometry assay. TG thapsigargin.

Back to article page