Fig. 5: SIRT1-mediated deacetylation of HINT1 promotes its tumor-suppressive function in colon cancer cell lines. | Experimental & Molecular Medicine

Fig. 5: SIRT1-mediated deacetylation of HINT1 promotes its tumor-suppressive function in colon cancer cell lines.

From: Deacetylation by SIRT1 promotes the tumor-suppressive activity of HINT1 by enhancing its binding capacity for β-catenin or MITF in colon cancer and melanoma cells

Fig. 5

DLD1 and SW480 cells were infected with control or Flag-HINT1 WT or 2KR-expressing lentivirus for 24 h. Lentiviral-infected DLD1 (a, c) and SW480 cells (b, d) were grown in a 96-well culture plate, and then cell counting was performed (a, b) and MTS assay (c, d) at the indicated times to determine cell proliferation rates. The same lentiviral-infected cell lines were grown for 14 days. The colonies formed by DLD1 (e) and SW480 cells (f) were stained and counted. Representative images are shown. Each Flag-SIRT1 WT or HY-expressing construct was transfected alone (g) or cotransfected (h) with the HINT1 WT construct into DLD1 cells, and then cell counting was performed to determine cell proliferation rates. All data are the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01.

Back to article page