Fig. 7: Deacetylation of HINT1 by SIRT1 also promotes its inhibition of MITF to suppress cell growth in A375 cell lines. | Experimental & Molecular Medicine

Fig. 7: Deacetylation of HINT1 by SIRT1 also promotes its inhibition of MITF to suppress cell growth in A375 cell lines.

From: Deacetylation by SIRT1 promotes the tumor-suppressive activity of HINT1 by enhancing its binding capacity for β-catenin or MITF in colon cancer and melanoma cells

Fig. 7

A375 cells were transfected with Flag-HINT1 WT or 2KR mutant and subjected to IP and western blot assays using antibodies as indicated. b A375 cells were transfected with the Flag-SIRT1 WT or HY mutant and subjected to IP and western blot assays using antibodies as indicated. c A375 cells were transfected with Flag-HINT1 WT or 2KR vectors and subjected to qRT-PCR, and western blot assays to determine the mRNA and protein levels of tyrosinase and Cdk2. d, e A375 cells were transfected with Flag-HINT1 WT or 2KR vectors followed by cell counting (d) and MTS assay (e) at the indicated times. f Flag-SIRT1 WT or HY was expressed in A375 cells, and then cell counting was performed to determine cell proliferation rates. g Flag-SIRT1 WT or HY constructs were cotransfected with the HINT1 WT construct in A375 cells, and then cell counting was performed to determine cell proliferation rates, and western blotting was performed to confirm the expression levels of expression vectors as indicated. h Proposed model depicting how reversible acetylation of HINT1 by SIRT1 and CBP regulates its tumor-suppressive activity in cancer cells. All data are the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01.

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