Fig. 1: Expression of Rad50 in pluripotent cells.
From: Rad50 mediates DNA demethylation to establish pluripotent reprogramming
a Quantitative real-time PCR analysis of Oct4 and Rad50 in MEFs, mESCs, and miPSCs. Data are expressed as the mean ± SEM. **p < 0.01, ANOVA with Tukey’s post hoc test. b Quantitative real-time PCR analysis of Oct4 and Rad50 at the 8-cell stage, 16-cell stage, and E3.5 blastocyst-stage mouse embryos. Data are expressed as the mean ± SEM. **p < 0.01, ANOVA with Tukey’s post hoc test. c Immunohistochemistry for Oct4 (Red), Rad50 (green), and DAPI (blue) in mESCs and blastocyst-stage embryos. d Western blot analysis for Rad50 and Oct4 in MEFs and mESCs. e Quantitative real-time PCR analysis of Oct4, Nanog, and Rad50 expression in mESCs following LIF withdrawal. f Quantitative real-time PCR analysis of Oct4 and Rad50 in mESCs following the addition of siOct4. Data are expressed as the mean ± SEM. **p < 0.01, ANOVA with Tukey’s post hoc test. The images (c, d) are representative of ≥3 similar experiments.
