Fig. 2: Rad50 induction of global DNA demethylation.
From: Rad50 mediates DNA demethylation to establish pluripotent reprogramming
a Dot blot analysis of 5mC and 5hmC in diluted genomic DNA from MEFs with Rad50 overexpression or knockdown. The image is representative of ≥3 similar experiments. b Average profile read mapping densities at the TSS determined by MBD-seq. c Average profile read mapping densities at the exon determined by MBD-seq. d Annotation of the differentially hypermethylated CpG islands, promoter, exon, and intron region following Rad50 overexpression or knockdown. e Gene ontology (GO) enrichment analysis of the hypermethylated region following knockdown of Rad50. f Bisulfite sequencing of the Oct4 and Nanog promoter regions in MEFs, mESCs, and Rad50-overexpressing MEFs.
