Fig. 3: Rad50 enhancement of OSKM-induced reprogramming efficiency.
From: Rad50 mediates DNA demethylation to establish pluripotent reprogramming
a Quantitative real-time PCR analysis of Oct4 and Rad50 expression during OSKM-mediated somatic cell reprogramming. Data are expressed as the mean ± SEM. *p < 0.05 and **p < 0.01, ANOVA with Tukey’s post hoc test. b Number of AP + colonies after OSKM-mediated somatic cell reprogramming. c Immunohistochemistry for Nanog (red), Oct4 (green), and DAPI (blue) following Rad50 overexpression or knockdown during OSKM reprogramming. d Quantitative real-time PCR analysis of pluripotent markers. Data are expressed as the mean ± SEM. **p < 0.01, ANOVA with Tukey’s post hoc test. e Morphology and fluorescence profiles of Oct4-GFP fibroblasts overexpressing Rad50. f The number of Oct4-GFP-positive colonies was counted during OSKM reprogramming. g Dot blot analysis of 5mC in diluted genomic DNA from cells exhibiting Rad50 overexpression or knockdown at day 15 during OSKM reprogramming. Data are expressed as the mean ± SEM. **p < 0.01, ANOVA with Tukey’s post hoc test. The images (c, e, and g) are representative of ≥3 similar experiments.
