Fig. 4: Rad50 interactions with Tet1.
From: Rad50 mediates DNA demethylation to establish pluripotent reprogramming
a Volcano plot reveals genes upregulated or downregulated by Rad50 overexpression, as determined by RNA-seq analysis. The threshold for determining differentially upregulated gene expression is indicated by a red box (Log(P value) > 2, Log(FoldChange) > 0.3). b GO enrichment analysis of significant upregulation as a result of Rad50 overexpression, as determined by RNA-seq analysis. c Venn diagram of the distribution of GO categories, cellular metabolic process, regulation of gene expression, chromosome organization, and chromatin organization, in Fig. 4b. d Quantitative real-time PCR validation of selected genes detected by RNA-seq analysis. Data are expressed as the mean ± SEM, n = 3. *p < 0.05 and **p < 0.01, two-sided Student’s t test. e Venn diagram showing overlapping binding regions shared between Rad50 and Tet1, as determined by Chip-seq analysis. f GO enrichment analysis of overlapping binding regions shared between Rad50 and Tet1.
