Fig. 6: l-CDL suppressed dopamine D1–D2DR complex formation and decreased the intracellular calcium concentration in spinal neurons. | Experimental & Molecular Medicine

Fig. 6: l-CDL suppressed dopamine D1–D2DR complex formation and decreased the intracellular calcium concentration in spinal neurons.

From: The dopamine D1–D2DR complex in the rat spinal cord promotes neuropathic pain by increasing neuronal excitability after chronic constriction injury

Fig. 6

a, b Coimmunoprecipitation of D1DR and D2DR in the spinal cords of CCI rats after intrathecal injection of l-CDL (15 μg/20 μL, i.t.). c, d Immunofluorescence showing the calcium concentration after administration of l-CDL (10 μM) and the effect of l-CDL (10 μM) on SKF 38393 (30 μM), quinpirole (30 μM), and SKF 83959 (30 μM)-induced augmentation of the calcium concentration in cultured spinal neurons. e, f Immunofluorescence showing the colocalization of D1DR and D2DR after administration of l-CDL (10 μM) and the effect of l-CDL on SKF 38393 (30 μM), quinpirole (30 μM), and SKF 83959 (30 μM)-induced increases in colocalization in the HEK293 cell membrane (n = 4; #P < 0.05, ##P < 0.01 vs the control group; **P < 0.01, ***P < 0.001 vs the SKF 38393 group; $$P < 0.01, $$$P < 0.001 vs the quinpirole group; &&&P < 0.001 vs the SKF 83959 group).

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