Fig. 2: Posthypoxic mRNA levels of proteins involved in the Sima pathway in Canton-S and Oregon-R D.m. after exposure to 2.5 h of hypoxia. | Experimental & Molecular Medicine

Fig. 2: Posthypoxic mRNA levels of proteins involved in the Sima pathway in Canton-S and Oregon-R D.m. after exposure to 2.5 h of hypoxia.

From: Posthypoxic behavioral impairment and mortality of Drosophila melanogaster are associated with high temperatures, enhanced predeath activity and oxidative stress

Fig. 2

a Schematic illustration of the Sima cascade. mRNA levels of Sima (b, c), Tango (d, e), Ldh (f, g), and iNOS (h, i) in both fly strains were evaluated at different posthypoxic reperfusion timepoints (0, 3, 6, 24, and 120 h after hypoxia). The target gene levels are presented as ratios to the levels of the constitutive genes Act5c and eEF1alpha2 and were normalized to the corresponding normoxia values. The graphs show the means ± SEMs from 3 independent experiments including 180 male flies per genotype for each treatment. Kruskal–Wallis test followed by Dunn’s multiple comparison test. *p < 0.05.

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