Fig. 9: Impacts of different reperfusion and hypoxia temperatures on the mortality rates of Canton-S and Oregon-R D.m.

a Schematic illustration of the experimental procedure used to assess the effects of different hypoxia temperatures on survival. Hypoxia of different durations (3–5 h) was performed at 18 °C, 23 °C, and 29 °C followed by 120 h of reperfusion at 29 °C. The death rates of Canton-S D.m. and Oregon-R D.m. after b 3 h, c 4 h, and d 5 h of severe hypoxia (<0.3% O₂) at 18 °C, 23 °C, and 29 °C followed by 120 h of reperfusion were determined. e Schematic illustration of the experimental procedure. In brief, hypoxia was performed at 29 °C for 3 to 5 h followed by 120 h of reperfusion at 18 °C, 23 °C, and 29 °C. The death rates of Canton-S D.m. and Oregon-R D.m. after f 3 h, g 4 h, and h 5 h of severe hypoxia (<0.3% O₂) followed by 120 h of reperfusion at 18 °C, 23 °C, and 29 °C were determined. The data are shown as the means ± SEMs of 3 independent experiments including a total of 60 male flies per genotype for each hypoxia duration and temperature.