Fig. 2: Cyclin D1 promoter activity and SOX9 binding elements in the Cyclin D1 promoter were affected by sublytic C5b-9 or the expression of SOX9 and ERK1/2. | Experimental & Molecular Medicine

Fig. 2: Cyclin D1 promoter activity and SOX9 binding elements in the Cyclin D1 promoter were affected by sublytic C5b-9 or the expression of SOX9 and ERK1/2.

From: Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis

Fig. 2

a Scheme showing full-length (FL) and the three indicated deletion mutants of the pGL-Cyclin D1 promoter according to JASPAR prediction. b–f Rat GMCs were cultured with various treatments or transfected with different plasmids with or without sublytic C5b-9 stimulation for 3 h. The activity of the Cyclin D1 promoter and the binding elements of SOX9 were measured by luciferase reporter assay (b–d) and ChIP-PCR (e, f). **p < 0.01 versus the MEM, Thy-1 Ab, Thy-1 Ab + HIS, or Thy-1 Ab + C6DS group or versus the pcDNA3.1, shCTR, or pGL3-basic + sublytic C5b-9 group; ΔΔp < 0.01 versus the shCTR + sublytic C5b-9, FL + sublytic C5b-9, -985 to +130 nt + sublytic C5b-9, or -582 to +130 nt + sublytic C5b-9 group. Data were represented as the means ± SD (n = 3 in each group in all experiments).

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