Fig. 5: Contribution of ERK1/2 activation to SOX9 phosphorylation, acetylation and nuclear expression. | Experimental & Molecular Medicine

Fig. 5: Contribution of ERK1/2 activation to SOX9 phosphorylation, acetylation and nuclear expression.

From: Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis

Fig. 5

a, c Rat GMCs overexpressing the pcDNA3.1-SOX9 and pcDNA3.1-ERK1/2 plasmids were cultured for 48 h. b, d In addition, GMCs were stimulated with sublytic C5b-9 for 3 h after transfection with the shERK1/2 plasmid. The interaction of SOX9 with p-ERK1/2 and the SOX9 acetylation level were measured by co-IP, and the SOX9 phosphorylation level was tested by IB (a, b). GMC cytoplasmic and nuclear proteins were isolated, and SOX9 was immunoprecipitated from the two fractions followed by IB with SOX9 and p-ERK1/2 antibodies (c, d). *p < 0.05, **p < 0.01 versus the shCTR group or nuclear extract; Δp < 0.05, ΔΔp < 0.01 versus the pcDNA-SOX9, pcDNA-ERK1/2, or shCTR + sublytic C5b-9 group. Data were represented as the means ± SD (n = 3 in each group in all experiments).

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