Fig. 6: The dependency of acetylated SOX9 on SOX9 phosphorylation for Cyclin D1 gene induction and GMC proliferation in response to sublytic C5b-9. | Experimental & Molecular Medicine

Fig. 6: The dependency of acetylated SOX9 on SOX9 phosphorylation for Cyclin D1 gene induction and GMC proliferation in response to sublytic C5b-9.

From: Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis

Fig. 6

Rat GMCs pretransfected with shSOX9 plasmid were treated with TSA (20 μM) for 30 min, followed by sublytic C5b-9 stimulation for 24 h or 3 h. GMC proliferation was determined by CCK-8 (a) and EdU incorporation assays (b). Cyclin D1 promoter activity and occupation by SOX9 were analyzed by luciferase reporter assay (c) and ChIP-PCR (d). The levels of SOX9 acetylation and phosphorylation and the protein expression of Cyclin D1 were determined by co-IP and IB (e). **p < 0.01 versus the shCTR + DMSO group; Δp < 0.05 or ΔΔp < 0.01 versus the shCTR + DMSO + sublytic C5b-9 group; ##p < 0.01 versus the shCTR + TSA + sublytic C5b-9 group. Data were represented as the means ± SD (n = 5 in each group for CCK‐8 assays, n = 3 in each group in the other experiments).

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