Fig. 4: Involvement of 4-1BB upregulation in the defective osteogenic differentiation potential of BMSCs from aged mice. | Experimental & Molecular Medicine

Fig. 4: Involvement of 4-1BB upregulation in the defective osteogenic differentiation potential of BMSCs from aged mice.

From: Activation of 4-1BB signaling in bone marrow stromal cells triggers bone loss via the p-38 MAPK-DKK1 axis in aged mice

Fig. 4

a ALP staining of BMSCs at week 1 after osteogenic induction with and without 4-1BBL treatment. b mRNA expression of osteogenic differentiation markers including Runx2, Opn, Bsp, and Ocn in BMSCs with and without 4-1BBL treatment was determined by qPCR. c Alizarin red staining of BMSCs at week 4 after osteogenic induction with and without 4-1BBL treatment. d ALP staining of BMSCs at week 1 after osteogenic induction with and without neutralization antibody against 4-1BB. e mRNA expression of osteogenic differentiation markers including Runx2, Opn, Bsp, and Ocn in BMSCs with and without neutralization antibody against 4-1BB. f Alizarin red staining of BMSCs at week 4 after osteogenic induction with and without neutralization antibody against 4-1BB. β-Actin was used as an internal control in qPCR. All the data were obtained from three independent experiments. Data are shown as the means ± SEMs. *p < 0.05, **p < 0.01, ***p < 0.001.

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