Fig. 5: DKK1 upregulation by 4-1BB via p38 MAPK in BMSCs from aged mice. | Experimental & Molecular Medicine

Fig. 5: DKK1 upregulation by 4-1BB via p38 MAPK in BMSCs from aged mice.

From: Activation of 4-1BB signaling in bone marrow stromal cells triggers bone loss via the p-38 MAPK-DKK1 axis in aged mice

Fig. 5

a DKK1 mRNA expression in BMSCs from 18-month-old mice in response to 4-1BBL and/or neutralization antibody against 4-1BB for 12 h was determined by qPCR. b DKK1 protein expression in BMSCs from 18-month-old mice in response to 4-1BBL and/or neutralization antibody against 4-1BB for 12 h was determined by western blot. c Active β-catenin protein expression in BMSCs from 18-month-old mice in response to 4-1BBL and/or neutralization antibody against 4-1BB for 12 h. d mRNA expression of Cyclin D1 and Axin2 in BMSCs from 18-month-old mice in response to 4-1BBL and/or neutralization antibody against 4-1BB for 12 h. e Phospho-p38 MAPK protein expression in BMSCs from 18-month-old mice in response to 4-1BBL and/or neutralization antibody against 4-1BB for 6 h. f Phospho-p38 MAPK protein expression in BMSCs from 18-month-old mice in response to 4-1BBL and/or gallocyanine treatment for 6 h. g DKK1 protein expression in BMSCs from 18-month-old mice in response to 4-1BBL and/or SB203580 treatment for 12 h. h ALP staining of BMSCs at week 1 after osteogenic induction with and without gallocyanine treatment. i mRNA expression of osteogenic differentiation markers including Runx2, Opn, Bsp, and Ocn in BMSCs with and without gallocyanine treatment. j Alizarin red staining of BMSCs at week 4 after osteogenic induction with and without gallocyanine treatment. All the data were obtained from three independent experiments. Data are shown as the means ± SEMs. *p < 0.05, **p < 0.01, ***p < 0.001.

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