Fig. 6: Rescue of trabecular bone loss in aged mice through in vivo blockade of 4-1BB signaling. | Experimental & Molecular Medicine

Fig. 6: Rescue of trabecular bone loss in aged mice through in vivo blockade of 4-1BB signaling.

From: Activation of 4-1BB signaling in bone marrow stromal cells triggers bone loss via the p-38 MAPK-DKK1 axis in aged mice

Fig. 6

a Experimental procedure. b BV/TV, Tb.N, and Tb.Sp of L1 vertebrae from 18-month-old mice with and without neutralization antibody against 4-1BB were determined by micro-CT. c Representative 3D reconstruction images of the L1 vertebrae Tb. d BV/TV, Tb.N, and Tb.Sp of the distal femur. e Representative 3D reconstruction images of the distal femur Tb. f Ct.ar/Tt.ar and Ct.Th of cortical bone in the femur mid-shaft. g Representative 3D reconstruction images of femur Ct. h Double calcein labeling images and bone formation rate (BFR) quantification of 18-month-old mice with and without neutralization antibody against 4-1BB. i Trap staining images and Oc.S/BS quantification of 18-month-old mice with and without neutralization antibody against 4-1BB. j Serum levels of the bone turnover markers PINP and Trap-5b from 18-month-old mice with and without neutralization antibody against 4-1BB were determined by ELISA. β-Actin was used as an internal control in qPCR. All the data were obtained from three independent experiments. Data are shown as the means ± SEMs. *p < 0.05, **p < 0.01, ***p < 0.001.

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