Fig. 1: Ectopic expression of STAT5A inhibits BMSC adipogenesis in vitro. | Experimental & Molecular Medicine

Fig. 1: Ectopic expression of STAT5A inhibits BMSC adipogenesis in vitro.

From: Alternative regulatory mechanism for the maintenance of bone homeostasis via STAT5-mediated regulation of the differentiation of BMSCs into adipocytes

Fig. 1

a–c BMSCs were transduced with control (pMX-IRES-EGFP) or constitutively active STAT5A (pMX-STAT5A1*6-IRES-EGFP) retroviral vectors and cultured in the presence or absence of adipogenic differentiation factors (insulin, rosiglitazone, dexamethasone, and IBMX) for 5 days. a Cultured cells were stained using Oil Red O solution and Nile Red solution. b The ratio of GFP/Oil Red O double-positive adipocytes to Oil Red O-positive adipocytes was calculated. ***P < 0.001 vs. control; n.s. not significant. Statistical analyses were performed via Student’s t test. c The mRNA levels of Pparɣ, Cebpα, Fabp4, and Stat5a were assessed by quantitative real-time PCR. The data are presented as the mean ± SD of triplicate samples. **P < 0.01; ***P < 0.001 vs. control; n.s. not significant. Statistical analyses were performed via Student’s t test. AGM Adipogenic differentiation medium. Bar: (a) 100 µm.

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