Fig. 3: Methods using organoids to study infections.
From: Organoids as host models for infection biology – a review of methods
a Infection models using organoids: in their intact 3D structural form with apical or basolateral application of the infectious agent, inside-out infection, shredded organoids cocultured with pathogens and single-cell coculture infection. b Organoid-derived 2D monolayer infection models: primary cells with ECM coating, B cell transfer infection, apical or basolateral transwell infection. c Tissue engineering infection models seeded with organoid-derived primary cells: tissue scaffold infection, bioreactor perfusion infection, and organ-on-a-chip perfusion infection.
