Fig. 1: Organoid culture methods.
From: Gastrointestinal cancer organoids—applications in basic and translational cancer research
a Adult stem cell-derived epithelial organoids are embedded in a laminin-rich extracellular matrix-like (Matrigel® or BME®) and overlaid with growth factor-supplemented medium. b In the air-liquid-interface (ALI) organoid cultivation approach, epithelial tissue is cultivated with the surrounding stroma, including fibroblasts and immune cells. The tissue is grown in a type I collagen matrix on top of a transwell insert containing a permeable membrane providing the cells with growth medium. c Pluripotent stem cell (PSC)-derived organoids are generated by a stepwise differentiation protocol that guides PSCs into the tissue of interest. PSC-derived organoids are also embedded in Matrigel® or BME® and overlaid with growth factor-supplemented medium.
