Fig. 2: KDM4A increases HIF1α expression through its demethylase function. | Experimental & Molecular Medicine

Fig. 2: KDM4A increases HIF1α expression through its demethylase function.

From: Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion

Fig. 2

a RT-qPCR detection of HIF1α mRNA expression in clinical NPC tissues and adjacent tissues. b Western blot analysis of HIF1α protein expression in clinical NPC tissues and adjacent tissues. c Pearson correlation analysis of HIF1α mRNA expression with KDM4A mRNA expression. d Detection of the efficiency of KDM4A mRNA silencing by RT-qPCR. e ChIP detection of H3K9me3 enrichment in the HIF1α promoter region in each group of cells. f RT-qPCR detection of KDM4A and HIF1α mRNA expression in each group of cells. g Western blot analysis of KDM4A, H3K9me3, and HIF1α protein expression in each group of cells. *p < 0.05 vs. control cells or cells treated with si-NC or cells treated with DMSO. The measurement data are expressed as the mean ± standard deviation. Data between cancer tissues and adjacent tissues were compared using paired t tests. One-way ANOVA was used for multigroup comparisons. For patients, n = 55. The experiment was repeated three times independently.

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