Fig. 1: Discovery of novel MI-related factors using proteomic and network analyses. | Experimental & Molecular Medicine

Fig. 1: Discovery of novel MI-related factors using proteomic and network analyses.

From: MicroRNA-26a/b-5p promotes myocardial infarction-induced cell death by downregulating cytochrome c oxidase 5a

Fig. 1

a Schematic showing the experimental procedure of our study. b Representative silver-stained 2-DE gel images and c networks generated by STRING database analysis using differentially expressed factors in the hearts of control and MI rats. d Magnified images of the 2-DE gels and e validated results for cytochrome c oxidase 5a (Cox5a) protein expression in each group by immunoblot analysis. 2-DE experiments were performed in triplicate for each individual, and immunoblot experiments were carried out twice independently. Spot and band intensities were measured as area densities and analyzed using ImageJ software. The relative intensity level of an immunoblot band indicates the target protein level normalized to the β-actin level. Significant differences between groups were determined via ANOVA, with p values indicated as *p < 0.05 and **p < 0.01. CON control rat group, MI myocardial infarction rat group. n = 3 rats per group.

Back to article page