Fig. 4: Cox5a expression in H9c2 cells under normoxic and hypoxic conditions.

a Changes in the Cox5a level in total cells according to the hypoxic environment exposure time (3, 6, and 12 h) and b the mitochondrial Cox5a expression level 12 h after exposure to hypoxia were measured via immunoblot analysis; β-actin and VDAC were used as internal controls in total cells and mitochondria, respectively. c Changes in Cox5a expression after 12 h of incubation under normoxic and hypoxic conditions were investigated using immunocytochemical staining. Nuclei were stained with DAPI. Scale bar = 50 μm. The data are representative of two independent experiments.