Fig. 1: Identification of cell clusters in mouse ascending aortas by scRNA-seq. | Experimental & Molecular Medicine

Fig. 1: Identification of cell clusters in mouse ascending aortas by scRNA-seq.

From: Single-cell transcriptome analysis reveals cellular heterogeneity in the ascending aortas of normal and high-fat diet-fed mice

Fig. 1

a Schematic diagram of the scRNA-seq program. ND, normal diet; HFD, high-fat diet. b t-SNE plot of aggregate cells from ND and HFD mice (after quality control, there were 14,663 cells in the ND group and 9338 in the HFD group). c t-SNE visualization of clustering revealing 27 cell clusters. d t-SNE plots of cell clusters across the indicated conditions. e Dot plot of conserved marker genes per cluster. Fibro, fibroblasts; SMC, smooth muscle cells; EC, endothelial cells; Mo/MΦ, monocytes/macrophages; T, T cells; DC, dendritic cells; Neural, neural cells; B, B cells. f t-SNE plot of cell clusters. g Major cell types and corresponding marker genes. h Relative expression of several marker genes in all cells from all samples. The cells are projected onto t-SNE plots. i Bar chart of the relative proportions of cell types from aggregated ascending aortas.

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