Fig. 1: MHY-1685 attenuated the hCSC senescent phenotype.
a Schematic diagram of the experimental design. b After a long-term culture with vehicle or MHY-1685, the relative percentage of cell viability was measured by a CCK-8 assay (*p < 0.05 vs. vehicle). c hCSCs were continuously treated with vehicle or MHY-1685, and the proliferation rate of hCSCs was measured by a CCK-8 assay (*p < 0.05 vs. vehicle). d After treatment with vehicle or MHY-1685, the S-phase cells were quantified by a BrdU incorporation assay (*p < 0.05 vs. vehicle). e Expression of the cell cycle-related proteins Cyclin E, CDK2, Cyclin D1, and CDK4 as quantified by western blot assay (*p < 0.05 vs. vehicle). f Representative images of the cell morphology of hCSCs at the same passage. The cell length and width were measured and are presented as a graph (*p < 0.05 vs. vehicle). Scale bar: 100 µm. g After long-term culture with vehicle or MHY-1685, cellular senescence was quantified by SA-β-gal-positive cells (*p < 0.05 vs. vehicle). Scale bar: 50 µm. h Population doubling time of hCSCs with vehicle or MHY-1685 (*p < 0.05 vs. vehicle). i Heat map analysis of SASP-related genes after treatment with vehicle or MHY-1685. j Expression of P16INK4a, p53, and p27 as quantified by a western blot assay (*p < 0.05 vs. vehicle). Data are shown as the mean ± S.E.M.
