Fig. 1: Generation of GLA-mutant iPSC-derived kidney organoids with the CRISPR/Cas9 gene-editing system. | Experimental & Molecular Medicine

Fig. 1: Generation of GLA-mutant iPSC-derived kidney organoids with the CRISPR/Cas9 gene-editing system.

From: Human kidney organoids reveal the role of glutathione in Fabry disease

Fig. 1

a Representative brightfield images of the morphology of wild-type kidney organoids and GLA-knockout kidney organoids. Scale bar = 300 μm. b Representative western blot of the expression of GLA (Clones #5 and #9) in human iPSCs. c qRT–PCR analysis of GLA in human iPSCs. d Representative western blot of the expression of GLA (Clones #5 and #9) in human kidney organoids. e qRT–PCR analysis of GLA in human kidney organoids. f qRT–PCR analysis of WT1, ECAD, CDH16, PDGFR-β, and Pecam1 in human kidney organoids and GLA-mutant organoids. The values are the mean ± SEM. *p < 0.05; **p < 0.01.

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