Fig. 7: GSH treatment reduces oxidative stress in Fabry kidney organoids.
From: Human kidney organoids reveal the role of glutathione in Fabry disease
a GSH, b GSH/GSH + GSSG and c GPx were measured in GLA-mutant and GSH-treated GLA-mutant kidney organoids. d Immunofluorescence image showing the levels of ROS by staining with MitoSOX Red. Scale bar = 50 μm. e Quantification of ROS via analysis of the fluorescence intensity of MitoSOX Red. f Immunofluorescence image showing the levels of intracellular calcium by staining with Fluo-8. Scale bar = 50 μm. g Quantification of intracellular calcium via analysis of the fluorescence intensity of Fluo-8. h Immunofluorescence image showing mitochondrial dysfunction revealed by MitoTracker staining. Scale bar = 50 μm. i Quantification of mitochondrial levels via analysis of the fluorescence intensity of MitoTracker. The values are the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
