Fig. 2: AC008 inhibits chondrocyte viability and promotes chondrocyte apoptosis and ECM degradation. | Experimental & Molecular Medicine

Fig. 2: AC008 inhibits chondrocyte viability and promotes chondrocyte apoptosis and ECM degradation.

From: m6A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p‒AQP1/ANKH axis

Fig. 2

a qRT–PCR was performed to verify the overexpression and knockdown efficiencies of AC008 in chondrocytes transfected with pcDNA-AC008 or AC008 ASO. b A CCK-8 assay was used to identify the viability of chondrocytes with AC008 overexpression or knockdown. c Chondrocyte apoptosis was detected with FITC-Annexin V/PI double staining using flow cytometry following AC008 overexpression or knockdown. d The expression levels of apoptosis-associated proteins were evaluated by western blotting. e The expression levels of ECM proteins (Aggrecan and COL2A1) and cartilage-degrading enzymes (MMP13 and ADAMTS-5) were analyzed by western blotting in chondrocytes following AC008 overexpression or knockdown. The data are presented as the means ± SDs. Statistical differences were determined using unpaired two-tailed Student’s t test (a, c) or two-way ANOVA (b). *P < 0.05; **P < 0.01; ***P < 0.001.

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