Fig. 7: m6A modification is involved in the upregulation of AC008 in OA. | Experimental & Molecular Medicine

Fig. 7: m6A modification is involved in the upregulation of AC008 in OA.

From: m6A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p‒AQP1/ANKH axis

Fig. 7

a The m6A modification site in AC008 was predicted with the online tool SRAMP (http://www.cuilab.cn/sramp). b The m6A level of AC008 in chondrocytes was determined by MeRIP-qPCR. c The expression level of FTO in normal and osteoarthritic cartilages was evaluated by qRT-PCR (n = 39). d qRT-PCR was performed to verify the overexpression efficiency of FTO in chondrocytes transfected with the FTO overexpression plasmid. e The m6A level of AC008 was evaluated in chondrocytes transfected with the indicated plasmids. f The expression level of AC008 was evaluated in chondrocytes transfected with the indicated plasmids. g Cells were treated with actinomycin D for the indicated times, and qRT-PCR was performed to evaluate the expression level of AC008. h Proposed model of the mechanism underlying the expression and function of AC008 in OA progression. The data are presented as the means ± SDs. Statistical differences were determined using unpaired two-tailed Student’s t test (b–f). *P < 0.05; **P < 0.01; ***P < 0.001.

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