Fig. 2: BIX suppresses normal mitochondrial function. | Experimental & Molecular Medicine

Fig. 2: BIX suppresses normal mitochondrial function.

From: BIX01294 inhibits EGFR signaling in EGFR-mutant lung adenocarcinoma cells through a BCKDHA-mediated reduction in the EGFR level

Fig. 2

a EGFR-mutant NSCLC cells were treated with the indicated concentration of BIX for 48 h and assayed for intracellular ATP. b PC9 cells were treated with the indicated concentration of BIX for 48 h, and the oxygen consumption rate was measured with an extracellular flux analyzer. c TCA cycle metabolite pools in PC9 cells treated with BIX (10 µM) were analyzed using LC–MS/MS. d EGFR-mutant NSCLC cells were treated with BIX (10 µM) in the presence or absence of α-ketoglutarate (AKG; 2 mM) and immunoblotted with the indicated antibodies. The error bars indicate the s.d. of triplicate wells from a representative experiment; **p < 0.01.

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