Fig. 2: Tie1 silencing decreases the hCG-induced VEGF level in SVOG cells. | Experimental & Molecular Medicine

Fig. 2: Tie1 silencing decreases the hCG-induced VEGF level in SVOG cells.

From: Tie1 contributes to the development of ovarian hyperstimulation syndrome under the regulation of EGR1 in granulosa cells

Fig. 2

a, b Tie1 and VEGF levels in SVOG cells cultured in the absence or presence of 10 IU/mL hCG for the indicated periods of time, as determined by RT-qPCR (a) and western blotting (b). c Tie1 levels in mock (siR-con) and Tie1 (siR-Tie1)-silenced SVOG cells, as determined by western blotting. d, e VEGF levels in mock and Tie1-silenced SVOG cells cultured in the absence (−) or presence (+) of 10 IU/mL hCG for 24 h, as determined by RT-qPCR (d) and western blotting (e). β-Actin served as the internal control. The optical density units of each target protein were normalized to β-actin and are expressed as fold-change. The data from three independent experiments are expressed as arbitrary units using the mean ± SEM. *P < 0.05, **P < 0.01.

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