Fig. 3: PI3K-AKT signaling is involved in the hCG-induced, Tie1-mediated increase in VEGF levels. | Experimental & Molecular Medicine

Fig. 3: PI3K-AKT signaling is involved in the hCG-induced, Tie1-mediated increase in VEGF levels.

From: Tie1 contributes to the development of ovarian hyperstimulation syndrome under the regulation of EGR1 in granulosa cells

Fig. 3

a AKT and p-AKT levels in SVOG cells cultured in the absence or presence of 10 IU/mL hCG for the indicated periods of time, as determined by western blotting. b AKT and p-AKT levels in mock (siR-con) or Tie1 (siR-Tie1)-silenced SVOG cells cultured in the absence (−) or presence (+) of 10 IU/mL hCG for 24 h, as determined by western blotting. c AKT, p-AKT, and VEGF levels in SVOG cells pretreated with 20 μM LY294002 for 1 h, followed by culture in the absence or presence of 10 IU/mL hCG for 24 h, as determined by western blotting. β-Actin served as the internal control. The optical density units of each target protein were normalized to β-actin and are expressed as fold-change. The data from three independent experiments are expressed as arbitrary units using the mean ± SEM. *P < 0.05, **P < 0.01.

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