Fig. 4: HSP70 inhibited the mitochondrial apoptotic pathway. | Experimental & Molecular Medicine

Fig. 4: HSP70 inhibited the mitochondrial apoptotic pathway.

From: HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3

Fig. 4

a, b The MMP of NP cells measured by flow cytometry using JC-1 staining. c, d The production of mtROS in NP cells measured by flow cytometry using MitoSOX Red staining. e, f The production of cellular ROS in NP cells measured by flow cytometry using DCFH-DA staining. g The effects of TRC on ATP production in NP cells. h The MMP and production of mtROS and cellular ROS in NP cells measured by flow cytometry. i The effects of VER on ATP production in NP cells treated with TRC. j The effects of TRC on the expression of cleaved caspase 3, cleaved caspase 9, Bax, and Bcl-2. k The effects of VER on the expression of cleaved caspase 3, cleaved caspase 9, Bax, and Bcl-2 in NP cells treated with TRC. (N = 3, *P < 0.05; **P < 0.01; ***P < 0.001).

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