Fig. 6: HSP70 inhibited the mitochondrial apoptotic pathway by suppressing mitochondrial fission. | Experimental & Molecular Medicine

Fig. 6: HSP70 inhibited the mitochondrial apoptotic pathway by suppressing mitochondrial fission.

From: HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3

Fig. 6

a The effects of FCCP on the viability of NP cells treated with TRC (N ≥ 3). b Representative fluorescence photomicrograph of TOM20 examined by immunofluorescence staining of NP cells treated with TRC and FCCP (N = 3, original magnification: ×1000). c The MMP and the production of mtROS and cellular ROS in NP cells were measured by flow cytometry (N = 3). d The effects of FCCP on ATP production in NP cells treated with TRC (N = 3). e, f The effects of FCCP on apoptosis of NP cells measured by flow cytometry using Annexin V-FITC/PI staining (N = 3). g The effects of FCCP on the expression of cleaved caspase 3, cleaved caspase 9, Bax, and Bcl-2 in NP cells treated with TRC (N = 3). (**P < 0.01; ***P < 0.001 vs. the control group or the TRC+, FCCP 0 μM group, ###P < 0.001 vs. the TRC−, FCCP 0 μM group, NS not significant).

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