Fig. 7: HSP70 suppressed compression-induced mitochondrial fission by upregulating SIRT3 expression. | Experimental & Molecular Medicine

Fig. 7: HSP70 suppressed compression-induced mitochondrial fission by upregulating SIRT3 expression.

From: HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3

Fig. 7

a The effects of TRC on SIRT3 expression (N = 3). b Representative fluorescence photomicrograph of SIRT3 expression examined by immunofluorescence staining (N = 3, scale bar: 50 μm). c The effects of VER on SIRT3 expression in NP cells treated with TRC (N = 3). d Representative fluorescence photomicrograph of SIRT3 expression examined by immunofluorescence staining (N = 3, scale bar: 50 μm). e The effects of 3-TYP on the viability of NP cells treated with TRC (N ≥ 3). f The effects of 3-TYP on the expression of DRP1, MFF, Fis1, Mfn1, Mfn2, and OPA1 in NP cells treated with TRC (N = 3). g Representative fluorescence photomicrograph of TOM20 examined by immunofluorescence staining of NP cells treated with TRC and 3-TYP (N = 3, original magnification: ×1000). (*P < 0.05; **P < 0.01; ***P < 0.001 vs. the TRC+, 3-TYP 0 μM group, ##P < 0.01, ###P < 0.001 vs. the TRC−, 3-TYP 0 μM group).

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