Fig. 5: Differentiation potential of the CMKLR1+ MSC functional subpopulation. | Experimental & Molecular Medicine

Fig. 5: Differentiation potential of the CMKLR1+ MSC functional subpopulation.

From: Single-cell RNA sequencing analysis of human bone-marrow-derived mesenchymal stem cells and functional subpopulation identification

Fig. 5

a ARS results showed that the osteogenic differentiation ability of CMKLR1+ MSCs was stronger than that of CMKLR1− MSCs. b ALP assays showed that the osteogenic differentiation ability of CMKLR1+ MSCs was stronger than that of CMKLR1− MSCs. c ORO staining and quantitative results demonstrated that the adipogenic differentiation ability of CMKLR1+ MSCs was weaker than that of CMKLR1− MSCs. d H&E and Masson staining showed that more new bone formation was observed in the CMKLR1+ MSC group than in the CMKLR1− MSC group in vivo. More OCN+ osteoblasts were also observed in the CMKLR1+ MSC group than in the CMKLR1− MSC group in vivo. e H&E staining and perilipin-1 staining showed that the number of adipocytes in the CMKLR1+ MSC group was less than that in the CMKLR1− MSC group in vivo.

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