Fig. 3: The expression of collagen is increased in RTN3-null mouse kidneys. | Experimental & Molecular Medicine

Fig. 3: The expression of collagen is increased in RTN3-null mouse kidneys.

From: Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells

Fig. 3

a Significantly differentially expressed genes between WT and RTN3-null mouse kidneys revealed by RNA-seq data. b KEGG pathway enrichment analysis of differentially expressed genes. The red square indicates the ECM−receptor interaction pathway. c mRNA levels of collagen-related genes in WT and RTN3-null mouse kidneys at 13 months as assessed by real-time PCR. d, e Western blot analysis showing the expression of collagen type I/III, COL4A5, RTN3, and β-actin in WT and RTN3-null mouse kidneys at 13 months. f mRNA levels of collagen-related genes in HEK293 cells transfected with si-Control and si-RTN3. g, h TEM analysis showing the glomerular basement membranes of WT and RTN3-null mice at 13 months.

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