Fig. 6: The mechanism of action of PP-007 relies on the induction of HO-1 in monocytes/macrophages to resolve lung inflammation in vivo. | Experimental & Molecular Medicine

Fig. 6: The mechanism of action of PP-007 relies on the induction of HO-1 in monocytes/macrophages to resolve lung inflammation in vivo.

From: Recruitment of monocytes primed to express heme oxygenase-1 ameliorates pathological lung inflammation in cystic fibrosis

Fig. 6

a Representative dot plots displaying the percentages of DsRed+ alveolar macrophages (AMs; CD11c+CD64+), neutrophils (CD11c−CD11b+CD24+Ly6G+), and other monocytes/macrophages (gran-CD11c-CD11b+) in the lung tissues of Cx3Cr1Cre/+ (top panel) and HO-1Cx3Cr1 (bottom panel) mice 24 h after LPS treatment. b qPCR of Hmox-1 in white blood cells and lung tissues and densitometric analysis of HO-1 protein levels in lung tissues from Cx3Cr1Cre/+ and HO-1Cx3Cr1 mice at 6 h after PP-007 treatment (320 mg/kg). Data are shown as the fold increase relative to vehicle-treated Cx3Cr1Cre/+ mice. HO-1Cx3Cr1 mice were pretreated with vehicle (n = 3) or PP-007 (n = 4) and then nebulized with PA-LPS as shown in Fig. 5a. Cx3Cr1Cre/+ mice (n = 3) were pretreated with vehicle and used as a control. c Densitometric analysis of HO-1 in lung tissue lysates. The intensities of HO-1 immunoreactive signals were measured and normalized to the β-actin intensity. d Numbers of neutrophils in the BALF and lung parenchyma assessed by flow cytometry. e Ratio of the percentage of Ly6C- to Ly6C+ mo-Ms in the lungs based on the gating strategy shown in Supplementary Fig. 3. f Cytokine concentration in the BALF. The graphs show the mean ± SEM. Statistical analyses were conducted using a two-tailed unpaired Student’s t test with unequal variance: *P ≤ 0.05.

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