Fig. 2: miR-200c directly targets HAS2. | Experimental & Molecular Medicine

Fig. 2: miR-200c directly targets HAS2.

From: Hyaluronan synthase 2, a target of miR-200c, promotes carbon tetrachloride-induced acute and chronic liver inflammation via regulation of CCL3 and CCL4

Fig. 2

a Prediction of miR-200c binding to the 3’UTR of human HAS2 mRNA. b The effect of miR-200c mimic treatment on the HAS2 mRNA level. c The effect of miR-200c inhibitor treatment on the HAS2 mRNA level. d The effect of miR-200c mimic treatment on pEZX-HAS2-3’UTR luciferase activity. Luciferase activity was assessed in HEK293A cells, LX-2 cells, and mouse HSCs transfected with the negative control mimic or miR-200c mimic in combination with pEZX-control or pEZX-HAS2-3’UTR. **P < 0.01 indicates a significant difference between the control mimic (or inhibitor) and miR-200c mimic (or inhibitor) (n = 3–6 per group). The data are presented as the mean ± SEM values. Significance was assessed by two-tailed Student’s t-test (b, c) and one-way ANOVA with Tukey’s post-hoc test (d).

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