Fig. 5: Low-molecular-weight HA elevates Ccl3, Ccl4, and Tlr2 mRNA expression in HSCs and Kupffer cells. | Experimental & Molecular Medicine

Fig. 5: Low-molecular-weight HA elevates Ccl3, Ccl4, and Tlr2 mRNA expression in HSCs and Kupffer cells.

From: Hyaluronan synthase 2, a target of miR-200c, promotes carbon tetrachloride-induced acute and chronic liver inflammation via regulation of CCL3 and CCL4

Fig. 5

a, b Ccl3, Ccl4, Tlr2, and Tlr4 mRNA expression in mouse hepatic stellate cells (HSCs). a Cells were treated with vehicle or with 100 or 200 μg/ml low-molecular-weight hyaluronan (LMW-HA) in combination with 10 μg/ml polymyxin B for 12 h. b Cells were treated with vehicle (Con) or 600 μg/ml high-molecular-weight hyaluronan (HMW-HA) in the presence of 10 μg/ml polymyxin B for 12 h. c, d qRT–PCR analysis of Ccl3, Ccl4, Tlr2, and Tlr4 mRNA expression in mouse Kupffer cells (KC). Cells were treated with vehicle, with 100 or 200 μg/ml LMW-HA, or with 600 μg/ml HMW-HA in combination with 10 μg/ml polymyxin B for 4 h. *P < 0.05 and **P < 0.01 indicate a significant difference versus the Con group (n = 3 per group). N.S. not significant. The data are presented as the mean ± SEM values. Significance was assessed by one-way ANOVA with Tukey’s post-hoc test (a, c) and two-tailed Student’s t-test (b, d).

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