Fig. 2: Accumulation of polyUb-p53 during Cd exposure is independent of proteasomes. | Experimental & Molecular Medicine

Fig. 2: Accumulation of polyUb-p53 during Cd exposure is independent of proteasomes.

From: The role of autophagy in cadmium-induced acute toxicity in glomerular mesangial cells and tracking polyubiquitination of cytoplasmic p53 as a biomarker

Fig. 2

a–d Cells were treated with 23 µM Cd for 6 h and 12 h. Immunoblotting was performed on lysates for Ub, and 600 µg of remaining protein was used for IP analysis with a p53 antibody and mouse IgG and then immunoblotted for Ub (a, b), and vice versa (c, d). e, f MES13 cells were pretreated with increasing concentrations of MG132 (0.5–5 µM) for 2 h and then treated with Cd (23 µM) for 12 h, harvested, lysed, and immunoblotted for the indicated proteins. β-Actin was used as the loading control. The levels of HMW-p53 and monomer-p53 were quantified by densitometry and normalized to those of β-actin. The data are presented as the mean ± SD (n = 3). *P < 0.05; **P < 0.005; ***P < 0.0005.

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