Fig. 1: MiRNA-1224-5p accelerates bone repair by enhancing BMSC differentiation to osteoblasts. | Experimental & Molecular Medicine

Fig. 1: MiRNA-1224-5p accelerates bone repair by enhancing BMSC differentiation to osteoblasts.

From: MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2

Fig. 1

a1 The results of peripheral blood sequencing of patients showed that the expression of miR-1224-5p in the fracture group was significantly higher than that in the normal group (control patients vs. fracture patients = 3 vs. 3). a2 The differentially expressed miRNA heatmap shows the top 85 miRNAs with upregulated expression in the peripheral blood of the fracture group compared with those of the control patients (P < 0.001, log2FC is greater than or equal to 2). a3 The trend of miR-1224-5p expression in the peripheral blood of patients after fracture is consistent with the bone remodeling process of fracture healing. b1, b2 Alkaline phosphatase (ALP) staining showed that BMSCs were induced to osteoblasts for 2 weeks after miRNA transfection, and ALP staining in the agomR-1224-5p group was significantly enhanced. b3, b4 In the agomiR-1224-5p group, the expression of key osteogenic target proteins and mRNAs was significantly upregulated compared with that in the other groups. Scale bar, 500 µm. c1, c2 Alizarin red (ARS) staining results showed that BMSCs were induced to osteoblasts for 3 weeks after transfection, and the mineralized nodules in the agomR-1224-5p group were significantly enhanced. c3 The expression of key osteogenic target mRNAs in the agomiR-1224-5p treatment group was significantly upregulated compared with that in the other groups. Scale bar, 500 µm. d1, d2 The results of oil red O staining showed that BMSCs were induced to differentiate into adipocytes for 3 weeks after transfection, and the ability of agomR-1224-5p to form fat was significantly weakened. e1–4 After BMMs were transfected with miRNA and induced to differentiate into osteoclasts for 5 days, the number and size of osteoclasts in the agomiR-1224-5 group were significantly smaller than those in the other groups. d3, d4 The expression levels of osteoclast differentiation marker proteins and mRNAs in the agomiR-1224-5p group were lower than those in the other groups. f1–3 In the agomR-1224-5p group, BV/TV and BMD were higher than those in the control group and the antagomiR-1224-5p group. f4 The results of the local H&E sections of the fracture model showed that the continuity of the new callus at the fracture end of the agomiR-1224-5p group was better than that of the control group and the other groups. The results are presented as the mean ± standard deviation, N = 5; #P ≥ 0.05; *P < 0.05, **P < 0.01; ***P < 0.001 by analysis of variance (ANOVA) with Tukey’s post-hoc test.

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