Fig. 3: Overexpression of miR-1224-5p leads to bone formation in elderly adult mice. | Experimental & Molecular Medicine

Fig. 3: Overexpression of miR-1224-5p leads to bone formation in elderly adult mice.

From: MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2

Fig. 3

a MicroCT reconstruction of the representative distal femur. The trabeculae of the agomiRNA-1224 group were significantly increased. b H&E-stained decalcified distal femur section. The bone trabeculae of the agomiRNA-1224 group were significantly thicker, ordered, and increased. c TRAP staining of distal femoral sections. The TRAP-positive cells in the agomiRNA-1224 group were significantly fewer than those in the control group and the antagomiRNA-1224 group. d MicroCT reconstruction of the representative (4th lumbar) L4 vertebral body. The trabeculae of the agomiRNA-1224 group were significantly increased. e Morphological analysis of the femoral metaphysis. f Analysis of morphological results of vertebral body tissue. g Serum CTX and P1NP expression levels. The results are presented as the mean ± standard deviation, N = 5; #P ≥ 0.05; *P < 0.05, **P < 0.01; ***P < 0.001 by analysis of variance (ANOVA) with Tukey’s post-hoc test. Scale bar, 500 µm.

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