Fig. 4: MiR-1224-5p inhibits RANKL-induced osteoclastogenesis. | Experimental & Molecular Medicine

Fig. 4: MiR-1224-5p inhibits RANKL-induced osteoclastogenesis.

From: MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2

Fig. 4

a, b TRAP staining pictures of representative BMMs 5 days after osteoclast differentiation. c, d The number and area of bone resorption pits. e The results of the representative bone resorption pit experiment on bovine bone slices after 9 days of culture. f, g Area and number of absorption pits. h A representative picture of osteoclasts and osteoblasts cocultured for 5 days in medium containing 1,25(OH)2D3 and PGE2 after transfection with different miRNAs. i, j Number and size of osteoclasts. k–m Cell proliferation and apoptosis experiments. BMMs were transfected with different miRNAs for 6 h and cultured for 48 h with 30 ng/ml M-CSF and 50 ng/ml RANKL. The results are presented as the mean ± standard deviation, N = 5, scale bar, 500 µm.

Back to article page