Fig. 8: Differential cellular localizations in mitochondrial Lactate Oxidation Complexes (mLOC) proteins in normal and cancer breast cell lines.
From: Tracing the lactate shuttle to the mitochondrial reticulum
Immunohistochemical detection of LDH, MCT isoforms, and cytochrome oxidase (COx) in the control breast cell line HMEC-184, Plate A, left, and in the breast cancer cell line MCF-7, Plate B, right. LDH isoforms MCT2 and MCT4 colocalized with the mitochondrial protein marker COx (rows A, C, D) but not MCT1, which was localized mainly in the plasma membrane in the control HMEC cell line (Plate B, Row B, left). Thus, mMCT isoform expression in breast cell mitochondria differs from that in skeletal muscle, where MCT1 predominates (Figs. 4 and 5); in control and cancer breast cells, MCT-2 and -4 isoforms colocalized with the inner mitochondrial membrane component COx. The thickness of the optical sections, ~1 µm, scale bar = 1 µm. Images from Hussien and Brooks140.
