Fig. 2: The Set7_1a complex disrupts the SAM–SET7/9 interaction by engaging SET7/9. | Experimental & Molecular Medicine

Fig. 2: The Set7_1a complex disrupts the SAM–SET7/9 interaction by engaging SET7/9.

From: Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9

Fig. 2

a, b Western blot analysis to evaluate the effect of the Set7_1a complex (10 μM) on the stabilization of SET7/9 in cellulo. c FTS analysis to evaluate the stability of recombinant His-tagged SET7/9 protein in the presence or absence of Set7_1a (10 μM). d ITC titration of recombinant SET7/9 protein (500 μM) into Set7_1a (50 μM). e BLI sensorgrams of the interaction between Set7_1a and recombinant His-tagged SET7/9 protein. f Covalent binding of Hcy to Set7_1a was confirmed by mass spectrometry. The main adduct formed at 721.2821 (MS positive, apo-Set7_1a + Hcy). Apo = Apomictic. g The effect of Set7_1a with or without Hcy on the SAM-SET7/9 interaction in vitro was estimated using a fluorescence polarization assay. h The relative levels of SET7/9 in hyperglycemia-induced HUVECs after Ctr and SET7/9 siRNA treatment. i Effect of the Set7_1a complex on HIF-1α and SET7/9 levels in hyperglycemia-induced HUVECs with or without SET7/9 knockdown. *P < 0.05, **P < 0.01 vs. the Ctr group.

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