Fig. 7: PDE4D promotes apoptosis through Bad phosphorylation regulated by PKA. | Experimental & Molecular Medicine

Fig. 7: PDE4D promotes apoptosis through Bad phosphorylation regulated by PKA.

From: Phosphodiesterase 4D contributes to angiotensin II-induced abdominal aortic aneurysm through smooth muscle cell apoptosis

Fig. 7

a Representative immunoblot analysis of caspase-3 and cleaved caspase-3 expression in the SMCs treated with PKI (PKA inhibitor, 10 μM, 60 min) and/or Ang II (100 nM, 24 h) and/or PDE4D siRNA (200 nM, 48 h) as indicated. b Quantification of cleaved caspase-3 protein expression by immunoblotting in (a) normalized to caspase-3 protein (fold change versus control). **p < 0.01, ***p < 0.001, ****p < 0.0001, one-way ANOVA with Holm-Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. c Immunoblot analysis of caspase-3 and cleaved caspase-3 expression in the SMCs treated with ESI-09 (Epac inhibitor, 100 μM, 60 min) and/or Ang II (100 nM, 24 h) and/or PDE4D siRNA (200 nM, 48 h) as indicated. d Quantification of cleaved caspase-3 protein expression by immunoblotting in (c) normalized to caspase-3 protein (fold change versus control). ****p < 0.0001, ns: no significant difference, one-way ANOVA with Holm–Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. e Representative immunoblot analysis of phospho-Bad (pBad) and Bad expression in the SMCs treated with PKI (PKA inhibitor, 10 μM, 60 min) and/or Ang II (100 nM, 24 h) and/or PDE4D siRNA (200 nM, 48 h) as indicated. f Quantification of pBad expression by immunoblotting in (e) normalized to Bad protein (fold change versus control). **p < 0.01, ****p < 0.0001, one-way ANOVA with Holm-Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. g Quantification of pBad expression by immunoblotting in (e) normalized to GAPDH protein (fold change versus control). **p < 0.01, ***p < 0.001, one-way ANOVA with Holm–Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. h Quantification of Bad expression by immunoblotting in (e) normalized to GAPDH protein (fold change versus control). **p < 0.01, ***p < 0.001, ****p < 0.0001, one-way ANOVA with Holm-Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. i PDE4D expression was significantly increased in Ang II-induced AAA, aggravating SMC apoptosis, which promoted pBad via the cAMP-PKA axis.

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