Fig. 5: Deletion of Pld2 promotes actin ring formation and microtubule acetylation through Akt activation. | Experimental & Molecular Medicine

Fig. 5: Deletion of Pld2 promotes actin ring formation and microtubule acetylation through Akt activation.

From: Phospholipase D2 controls bone homeostasis by modulating M-CSF-dependent osteoclastic cell migration and microtubule stability

Fig. 5

a–d WT and Pld2−/− BMMs were cultured on coverslips for 5 days with M-CSF and RANKL. a Nuclei, F-actin, and tubulin in osteoclasts were stained with Hoechst (blue), TRITC-conjugated phalloidin (red), and anti-tubulin (green) antibodies, respectively, and detected using a fluorescence microscope. b Quantification of relative actin ring size. Data are expressed as the mean ± SD. *p < 0.05. c Osteoclasts were treated without or with nocodazole (1 μM) for 30 min, fixed, and labeled with anti-tubulin antibody. d Osteoclasts were stained with an anti-acetylated tubulin antibody. e, f Osteoclast lysates were immunoblotted with the indicated antibodies. g Osteoclasts were cultured without or with the Akt inhibitor MK2206 (1 μM) for 16 h, fixed, and labeled with FITC-conjugated phalloidin (green) and anti-acetylated tubulin (red) antibody. All scale bars represent 100 μm.

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